SpiderMan - S1E02 - The Spider-Slayers

In "The Spider Slayer," the Spider-Slayers being commissioned by Norman Osborn for hunting Spider-Man with Spencer Smythe at the controls. Unfortunately, the "Black Widow" Spider-Slayer was destroyed and the factory exploded with Spencer Smythe still in it. This culminated in Alistair Smythe working for the Kingpin when his father was presumed dead. In "Return of the Spider-Slayers," Alistair would go on to not only recreate the "Black Widow" Spider-Slayer, but also at the same time to unleash the "Tarantula" and "Scorpion" Spider-Slayers. In this depiction, the three Spider-Slayers could join together as a massive engine of destruction.